Research Experience

Research Experience Fellowships Publications

I am fascinated by the constant warfare between hosts and their pathogens. I particularly love neat imaging methods that study this battle in live single cells because I enjoy watching these dynamic processes.

Research Experience

(Photo by Casey A. Cass – University of Colorado)

At the University of Colorado Boulder, I studied Salmonella virulence proteins. I was curious about how they helped Salmonella infection in multiple cell types.

For this research, I developed a long-term imaging assay to track infections in single cells for up to 48 hours post infection using fluorescence microscopes. I also developed corresponding image analysis methods. For this work, I created several new Salmonella strains using standard Salmonella genetics methods and became an expert in many different image analysis programs (such as FIJI, Icy, and IMARIS). Furthermore, I gained limited knowledge in Python, Javascript and MatLab. As a graduate student, I received an NIH F31 grant for this project.

During this work, I found I wanted to learn more about fluorescence microscopes. I attended an Andor Academy workshop at the University of Colorado Boulder BioFrontiers Institute. I also attended Cold Spring Harbor Laboratory’s Quantitative Imaging course in April 2015.

My previous research includes studying how an Epstein-Barr virus protein affects cell signaling pathways, writing a computer program (in C) to model kinesin movement, using atomic force microscopy to study biofilm formation, using mass spectrometry to study transcriptional regulation in yeast, and studying how a human T cell leukemia virus protein affects transcription in mammalian cells.

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Fellowships and Travel Grants

As a graduate student, I applied for the following fellowships:

  • NIH F31 Predoctoral Fellowship (received and renewed)
  • Ford Dissertation Fellowship (received an honorable mention but not the grant)
  • NIH T32 Training Grant: Signaling and Cell Cycle Regulation (received)

I  also received these travel grants:

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Publications and Presentations

Publications
Young, A. M., Minson, M., McQuate, S. E., and Palmer, A.E. 2017. Optimized fluorescence complementation platform for visualizing Salmonella effector proteins reveals distinctly different intracellular niches in different cell types. ACS Infectious Diseases. epub ahead of print.Optimized Fluorescence Complementation Platform for Visualizing Salmonella Effector Proteins Reveals Distinctly Different Intracellular Niches in Different Cell Types.

McQuate, S. E., Young, A. M., Silva-Herzog, E., Bunker, E., Hernandez, M., de Chaumont, F., Liu, X., Detweiler, C.S., and Palmer, A.E. 2017. Long-Term Live Cell Imaging Reveals New Roles For Salmonella Effector Proteins SseG and SteA. Cellular Microbiology. 19, e12641. Long-term live-cell imaging reveals new roles for Salmonella effector proteins SseG and SteA.

Sammond, D.W., Joce, C., Takeshita, R., McQuate, S.E., Ghosh, N., Martin, J.M., and Yin, H. 2011. Transmembrane peptides used to investigate the homo-oligomeric interface and binding hotspot of latent membrane protein 1. Biopolymers. 95, 772-84.Transmembrane peptides used to investigate the homo-oligomeric interface and binding hotspot of latent membrane protein 1.

Oral Presentations
2014           Imaging Salmonella in live cells in order to elucidate the role of effector proteins in infection, Signaling and Cellular Regulation Annual Symposium, University of Colorado Boulder

Poster Presentations
2013           Using fluorescence microscopy tools to understand the role of SteA in Salmonella infection, poster presented at the following venues: Salmonella: The bacterium, the host and the environment ASM conference, Boston, MA; New Approaches and Concepts in Microbiology EMBO conference, Heidelberg, Germany.

2011           Visualizing Salmonella effectors in host cells and characterizing the intracellular niche, poster presented at American Society for Cell Biology annual meeting, Denver, CO.

2011           Visualizing Salmonella effectors in host cells and characterizing the intracellular niche, poster presented at Microbial Pathogenesis and Host Response Meeting, Cold Spring Harbor, NY.

2006           Deacetylation Levels on Histones H3 and H4 by Sir2 and Hos1 in Saccharomyces cerevisiae, poster presented at the following venues: Summer research poster session, University of Puget Sound, Tacoma, WA; M.J. Murdock Conference, University of Portland, Portland, OR; and the American Chemical Society National Meeting, Chicago, IL. (2007).

2005           The Effects of the HTLV-1 Tax Protein on the Nucleosome-occupancy in the Coding Region of Genes Transcribed by RNA Polymerase II, poster presented at REU program poster session, Colorado State University, Fort Collins, CO.

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