Research Experience

Research Experience Fellowships Publications

I am fascinated by the constant warfare between hosts and their pathogens. I particularly love novel imaging methods that study the host-pathogen interface in live single cells because I enjoy watching these dynamic processes.

Research Experience

(Photo by Casey A. Cass – University of Colorado)

At the University of Colorado Boulder, I studied Salmonella virulence proteins. I was curious to determine how they helped establish Salmonella  infection in multiple cell types.

For this research, I developed a long-term imaging assay to track infections in single cells for up to 48 hours post infection using confocal or widefield fluorescence microscopes. I also developed corresponding image analysis methods. For this work, I created several new Salmonella strains using standard Salmonella genetics methods and became an expert in many different image analysis programs (such as ImageJ, CellProfiler, ICY, and IMARIS). Furthermore, I gained limited knowledge in Python, Javascript and MatLab. As a graduate student, I received an NIH F31 grant for this project.

During this work, I found I wanted to learn more about fluorescence microscopes. I attended an Andor Academy workshop at the University of Colorado Boulder BioFrontiers Institute. I also attended Cold Spring Harbor Laboratory’s Quantitative Imaging course in April 2015.

My previous research includes studying how the oligomerization of an Epstein Barr Viral protein affects downstream signaling pathways, writing a computer program (in C) to model kinesin movement, using atomic force microscopy to study the effects of antibacterial polymers on biofilm formation, using mass spectrometry to study histone deacetylation patterns in yeast, and studying transcriptional regulation by a human T cell leukemia viral protein.

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Fellowships and Travel Grants

As a graduate student, I applied for the following fellowships:

  • NIH F31 Predoctoral Fellowship (received)
  • Ford Dissertation Fellowship (received an honorable mention but not the grant)
  • NIH T32 Training Grant: Signaling and Cell Cycle Regulation (received)

I  also received these travel grants:

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Publications and Presentations

Publications
McQuate, S. E., Young, A. M., Silva-Herzog, E., Bunker, E., Hernandez, M., de Chaumont, F., Liu, X., Detweiler, C.S., and Palmer, A.E. 2017. Long-Term Live Cell Imaging Reveals New Roles For Salmonella Effector Proteins SseG and SteA. Cellular Microbiology. 19, e12641.

Sammond, D.W., Joce, C., Takeshita, R., McQuate, S.E., Ghosh, N., Martin, J.M., and Yin, H. 2011. Transmembrane peptides used to investigate the homo-oligomeric interface and binding hotspot of latent membrane protein 1. Biopolymers. 95, 772-84.

Oral Presentations
2014           Imaging Salmonella in live cells in order to elucidate the role of effector proteins in infection, Signaling and Cellular Regulation Annual Symposium, University of Colorado Boulder

Poster Presentations
2013           Using fluorescence microscopy tools to understand the role of SteA in Salmonella infection, poster presented at the following venues: Salmonella: The bacterium, the host and the environment ASM conference, Boston, MA; New Approaches and Concepts in Microbiology EMBO conference, Heidelberg, Germany.

2011           Visualizing Salmonella effectors in host cells and characterizing the intracellular niche, poster presented at American Society for Cell Biology annual meeting, Denver, CO.

2011           Visualizing Salmonella effectors in host cells and characterizing the intracellular niche, poster presented at Microbial Pathogenesis and Host Response Meeting, Cold Spring Harbor, NY.

2006           Deacetylation Levels on Histones H3 and H4 by Sir2 and Hos1 in Saccharomyces cerevisiae, poster presented at the following venues: Summer research poster session, University of Puget Sound, Tacoma, WA; M.J. Murdock Conference, University of Portland, Portland, OR; and the American Chemical Society National Meeting, Chicago, IL. (2007).

2005           The Effects of the HTLV-1 Tax Protein on the Nucleosome-occupancy in the Coding Region of Genes Transcribed by RNA Polymerase II, poster presented at REU program poster session, Colorado State University, Fort Collins, CO.

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